PCR-based detection of a polymorphic HaeIII site in intron VII of the human albumin (ALB) gene.

نویسندگان

  • J C Moolman
  • V A Corfield
  • G Stevens
چکیده

Figure 1. DNA from an Icelandic family was digested with Xbal and hybridised withpl71.14, asubclone from a cosmid isolated with p212/9. Autoradiographic exposure was for 16 hours. PCR-based detection of a polymorphic Haelll site in intron VII of the human albumin (ALB) gene J.C.Moolman, V.A.Corfield and G.Stevens Medical Research Council/University of Stellenbosch Centre for Molecular and Cellular Biology, PO Box 19063, Tygerberg 7505 and MRC Ecogenetics Research Unit, Dept. of Human Genetics, School of Pathology, South African Institute of Medical Research and University of the Witwatersrand, Johannesburg, South Africa

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Efficiency of BclI Restriction Fragment Length Polymorphism for Detection of Hemophilia A Carriers in Sistan and Baluchestan Province, Southeast of Iran

Background: Indirect genetic diagnosis using polymorphic DNA markers can detect carriers of hemophilia A. This technique is preferable in developing countries because of its simplicity and cost effectiveness compared to direct mutation analysis. In the present study, we examined usefulness of intragenic marker BclI restriction fragment length polymorphism (RFLP) at intron 18, for carrier detect...

متن کامل

بررسی فراوانی پلی مورفیسم جایگاه SspI در اینترون II ژن بتا گلوبین در استان مازندران

Background and purpose: Due to the high annual birth rate of thalassemia major in our country, its prevention by prenatal diagnosis is of important priority. Gene mutation remains unknown in 10-20% of thalassemia trait people in Iran. In these cases, linkage analysis using polymorphic sites which are located near or within the gene is necessary to follow the mutant or the normal chromosome. Ssp...

متن کامل

Genotyping of Intron 22 and Intron 1 Inversions of Factor VIII Gene Using an Inverse-Shifting PCR Method in an Iranian Family with Severe Haemophilia A

Abstract Background: Haemophilia A (HA) is an X-linked bleeding disorder caused by the absence or reduced activity of coagulation factor VIII (FVIII). Coagulation factors are a group of related proteins that are essential for the formation of blood clots. The aim of this study was to genotype the coagulation factor VIII gene mutations using Inverse Shifting PCR (IS-PCR) in an Iranian family ...

متن کامل

Growth Hormone Gene Polymorphism in Two Iranian Native Fowls (Short Communication)

Biochemical polymorphism study is a method of determination of genetic variation. This variability could be a basis for selection and subsequent genetic improvement in farm animals. The polymorphism in the intron 1 of chicken growth hormone (cGH) gene was investigated in the Iranian native fowls by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. The...

متن کامل

P-122: The Effect of Beta Globin Intron on Human LH Hormone Expression in CHO Cells

Background Luteinizing hormone (LH) is a heterodimeric glycoprotein composed of alpha and beta subunits.This hormone is secreted from the pituitary gland. LH, in women triggers Menstrual cycle and ovulation. In men, LH stimulates production of testosterone, which plays a specialized role in sperm production. Up to day, LH hormone have produced in different ways such as codon optimization and al...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Nucleic acids research

دوره 19 24  شماره 

صفحات  -

تاریخ انتشار 1991